The group has been focused in the past 12 years on developing strategies for tissue regeneration for the pediatric patients and has acquired different skills in the field of stem cell biology, molecular biology and murine animal model creating a strong expertise on regenerative medicine field.
In particular, the research activity is focused on three different area:
- amniotic fluid stem cells and satellite cells, the skeletal muscle stem cell population: application in endothelial reconstruction and muscle disease models;
- extracellular matrix as tissue bulk and cell recipient. Decellularization methods of healthy skeletal muscle tissue or of origin (of murine and human source) are performed together with tissue engineering techniques to rebuilt the complexity of 3D tissues.
- decellularization of tumor tissue has been settled as tissue engineering technique to develop an approach of personalized translational medicine both for treatment and diagnostics. The introduction in oncology of a tool developed in the context of regenerative medicine such as decellularized matrix, is without doubts an enrichment of the scientist efforts in understanding the complex relationship between microenvironment, tumor growth and dissemination.
Since 2015 (when Prof. Muraca joined the group), research on extracellular vesicles (EVs) has been developed. EVs are nanometer-size particles secreted by different types of cells and contain proteins, growth factors, mRNA and other molecules. Prof. Muraca’s research activity and collaborations in this field gave rise to three patents:
- A patent on the immunomodulatory effect of EVs derived by mesenchymal stem cells (MSCs), acquired by the Company Esperite/CryoSave. The Company is collaborating with our Lab to develop clinical applications of this product
- A patent on EVs from the osteoblastic lineage as drug carriers with specific targeting to bone tissue, developed in collaboration with Professor Anna Teti and Dr. Andrea Cappariello
- A patent on the anti-angiogenic activity of EVs from cytokine-induced MSCs, developed in collaboration with Professor Antonella Viola.
- Annamaria Tolomeo
- Giovanna Spiro
- Mattia Saggioro
- Michele Grassi
- Caterina Trevisan
- Stefania D’Agostino
- Federica Slaviero
- Fabio Magarotto
Selected PublicationsPozzobon M, Saggioro M, D'Agostino S, Bisogno G, Muraca M, Gamba P.. 2017. Alveolar Rhabdomyosarcoma Decellularization. Methods Mol Biol, doi: 10.1007/7651_2017_45
Bertin E, Piccoli M, Franzin C, Spiro G, Donà S, Dedja A, Schiavi F, Taschin E, Bonaldo P, Braghetta P, De Coppi P, Pozzobon M. 2016. First steps to define murine amniotic fluid stem cell microenvironment. Sci Rep, 15;6:37080
Bertin E, Piccoli M, Franzin C, Nagy A, Mileikovsky M, De Coppi P, Pozzobon M.. 2015. Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system. Stem Cell Research, 15(3): 510-3
Schiavo AA, Franzin C, Albiero M, Piccoli M, Spiro G, Bertin E, Urbani L, Visentin S, Cosmi E, Fadini GP, De Coppi P, Pozzobon M.. 2015. Endothelial properties of third-trimester amniotic fluid stem cells cultured in hypoxia. Stem. Cell Res Ther, 31;6:209
Piccoli M, Urbani L, Alvarez-Fallas ME, Franzin C, Dedja A, Bertin E, Zuccolotto G, Rosato A, Pavan P, Elvassore N, De Coppi P, Pozzobon M.. 2015. Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch. Biomaterials, 74:245-255
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