Conventional in vitro models used to study human tissue pathophysiology often fail to mimic in vivo relevant cell behavior. However, the development of novel in vitro models that can overtake such limitations are needed to move research from animal models to patients. This is particularly evident for skeletal muscle, where myofiber contraction and homeostasis is also guaranteed by the combination of a proper myofiber 3D organization and by the action of the nervous system. Skeletal muscle can be functionally compromised due to dysfunction of muscular and/or of neuronal components, as in neuromuscular diseases.
Our recently established group results from the multidisciplinary integration of different expertise’s in skeletal muscle and stem cell biology, extracellular matrix and biomaterial engineering. Our current research project aims at generating 3D models of human skeletal muscle equipped with a neuronal network. Such 3D in vitro models will be used to mimic the structural/functional properties of human neuromuscular junction, for the identification of cellular and molecular players involved in neuromuscular genesis and maintenance in healthy and disease.
- Paolo Raffa
- Valentina Scattolini
Selected PublicationsUrciuolo A, Urbani L, Perin S, et al. . 2018. Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration. Scientific Reports, 8:8398
Brigo L, Urciuolo A, Giulitti S, Della Giustina G, Tromayer M, Liska R, Elvassore N, Giovanna Brusatin. 2017. 3D high-resolution two-photon crosslinked hydrogel structures for biological studies. Acta Biomater, 55:373-384
Gattazzo F, Urciuolo A & Bonaldo P.. 2014. Extracellular matrix: A dynamic microenvironment for stem cell niche. Biochim Biophys Acta, S0304-4165(14)00012-9.
Urciuolo A, Quarta M, Morbidoni V, Gattazzo F, Molon S, Grumati P, Montemurro F, Tedesco FS, Cossu G, Vozzi G, Rando TA, Bonaldo P. Collagen. 2013. VI regulates satellite cell self-renewal and muscle regeneration. Nat Commun, 4:1964
Grumati P, Coletto L, Sabatelli P, Cescon M, Angelin A, Blaauw B, Tiepolo T, Urciuolo A, Maraldi NM, Bernardi P, Sandri M, Bonaldo P. 2010. Autophagy is defective in collagen VI muscular dystrophies and its reactivation rescues myofiber degeneration. Nat Med, 16(11), 1313-20
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