Dr. Delogu’s laboratory focus on the translation of different nanomaterials in the Medical Scenario, with an emphasis on three pillars:
i) the revealing of nanomaterial immune compatibility and intrinsic immune-properties
ii) the development and assessment of nanomaterials for tissue engineering
iii) the study of nanomaterials applications in space biology.
i) In collaborations with international leaders in nanomaterial production and chemical characterization, the lab mainly focuses on carbon-based materials (CBMs) such as carbon nanotube fibers, graphene oxide, aminated graphene, exfoliated graphene, graphene nanoribbons. Graphene, thanks to its chemical and physical properties, is the most promising, versatile, and sustainable key enabling nanotechnology and Dr. Delogu is currently coordinating the G-IMMUNOMICS partnering project of the European Graphene Flagship, and Carbo-IMMAP financed under HORIZON 2020. The Lab contributed in opening new fascinating perspectives for CBMs in nanomedicine from drug delivery to imaging and as immunomodulators (Delogu LG et al. Bioconjugate Chem 2009; Delogu LG et al. Nanomedicine Lond 2012; Delogu LG et al. PNAS 2012; Pescatori M et al. Biomaterials. 2013). The study of immune cell interactions with nanomaterials is of fundamental importance for almost any translational medicine application. We demonstrated how the investigation of the immune cell reactions is a milestone for the safe exploitation of graphene and nanomaterials in general (Orecchioni M et al. JTM 2014; Orecchioni M et al. Theranostics 2015; Orecchioni M et al. Adv Drug Deliv Rev 2016). We are working to build a pipeline based on multi-omics approaches to predict and detect the immune-effects of multiple physical-chemical parameters of CBMs. We are employing high throughput approaches, such as genome-wide transcriptomic analysis, multiparametric flow, and single-cell mass cytometry. We aim at achieving a comprehensive and quantitative picture of carbon-based nanomaterials immune-activity and behavior selecting them for different biomedical applications including cancer therapy (Orecchioni M et al. Nature Communications 2017, Orecchioni M et al. Adv Health Care Mat 2016). We carry out our studies using a wide variety of in vitro, and ex vivo human cells and also mice and swine Moreover, within this context, we work on the development of functionalized graphene-based tools against cancer and myelomonocytic leukemia in particular (Russier J et al., Angewandte Chemie Int. Ed. 2017).
ii) It is well recognized that graphene-based tools, depending on chemical characteristics, can play a role in the differentiation of mesenchymal stem cells (MSCs). Moreover, it is well know how the immune activation is a key factor in bone regeneration and formation, as well as preventing bone loss. The lab is currently working on graphene-based tools and novel synthetic magnesium-doped hydroxyapatite/type I collagen scaffolds with different functionalizations able to boost the stem cell differentiation into osteoblasts. We are testing the different nanotools osteoinductivity properties in vitro, by co-colturing MSCs and immune cells, and in vivo in mice models. The lab is interested in developing new nano-strategies in the context of bone regeneration.
iii) One of the major problems of long space flights is the immune function dysregulation; we previously reported the possibility to take advantage of CBMs immune properties under microgravity conditions (Crescio C et al. Nanoscale 2014). We are finding new tools able to compensate the immune down-regulation in Space. Moreover, microgravity conditions are an excellent environment to understand inflammation mechanisms; we are working to apply that knowledge to medical needs on the hearth. Also, the ImmuneNano-lab is today part of the multidisciplinary European topical team on “Tissue Healing in Space: Techniques for promoting and monitoring tissue repair and regeneration” with the aim to develop non-invasive devices for the monitoring of inflammatory, proliferative and remodeling phase of wound healing and scar formation.
Selected PublicationsRussier R, Léon V, Orecchioni M, Hirata E, Virdis P, Fozza C, Sgarrella F, Cuniberti G, Prato M, Vazquez E, Bianco A and Delogu LG.. 2017. Few-Layer Graphene Kills Selectively Tumor Cells from Myelomonocytic Leukemia Patients. Angew. Chem,
Orecchioni M, Jasim DA, Pescatori M, Manetti R, Fozza C, Sgarrella F, Bedognetti D, Bianco A, Kostarelos K and Delogu LG.. 2016. Molecular and Genomic Impact of Large and Small Lateral Dimension Graphene Oxide Sheets on Human Immune Cells from Healthy Donors. Adv Healthc Mater,
Pescatori M, Bedognetti D, Venturelli E, Ménard-Moyon C, Bernardini C, Muresu E, Piana A, Maida G, Manetti R, Sgarrella F, Bianco A, Delogu LG.. 2013. Functionalized carbon nanotubes as immunomodulator systems. Biomaterials,
Delogu LG, Vidili G, Venturelli E, Ménard-Moyon C, Zoroddu MA, Pilo G, Nicolussi P, Ligios C, Bedognetti D, Sgarrella F, Manetti R, Bianco A.. 2012. Functionalized multiwalled carbon nanotubes as ultrasound contrast agents. Proc Natl Acad Sci U S A,
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